Coding
RepA

Part:BBa_K4674002:Design

Designed by: Chun Ying Yu   Group: iGEM23_CCU-Taiwan   (2023-10-08)


RepA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The RepA sequence in this part is codon optimized for E. Coli expression


Source

The Rep A is originated from the R-plasmid, pC194, in Staphylococcus aureus. Thr protein sequnece could be obstained as UniProtKB/Swiss-Prot: P03862.1.

References

Horinouchi,S. and Weisblum,B. Nucleotide sequence and functional map of pC194, a plasmid that specifies inducible chloramphenicol resistance JOURNAL J Bacteriol 150 (2), 815-825 (1982)